DNA Polymerase delta, catalytic subunit Antibody

Images

 
Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP2-16184] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase delta, catalytic subunit Antibody [NBP2-16184] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.39 mg/ml

Order Details

DNA Polymerase delta, catalytic subunit Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DNA polymerase delta. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (84%), Zebrafish (80%), Xenopus tropicalis (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.39 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Polymerase delta, catalytic subunit Antibody

  • CDC2 homolog
  • CDC2
  • DNA polymerase delta catalytic subunit
  • DNA polymerase subunit delta p125
  • DNA-directed polymerase delta 1
  • EC 2.7.7.7
  • POLD
  • polymerase (DNA directed), delta 1, catalytic subunit (125kD)
  • polymerase (DNA directed), delta 1, catalytic subunit 125kDa

Background

The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1, POLD2 (MIM 600815), POLD3 (MIM 611415), and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POLD1
Uniprot