DNA Polymerase beta Antibody (61)

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Immunohistochemistry: DNA Polymerase beta Antibody (61) [NB600-1026] - IHC analysis of DNA polymerase b formalin-fixed paraffin embedded human testis using NB600-1026.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IB, IHC, IHC-Fr, IHC-P
Clone
61
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

DNA Polymerase beta Antibody (61) Summary

Immunogen
BALB/C mice were injected with DNA polymerase beta protein.
Localization
Nuclear
Specificity
This is specific to a 39 kD protein known as DNA polymerase beta.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POLB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoblotting
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
We suggest an incubation period of 30 minutes at room temperature.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in immunoblotting reported in scientific literature (PMID 28559431).
Positive Control
Testis Lysate (NB820-59266)
Publications
Read Publications using NB600-1026.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for DNA Polymerase beta Antibody (61)

  • DNA pol beta
  • DNA polymerase beta subunit
  • DNA Polymerase beta
  • EC 2.7.7.7
  • EC 4.2.99.-
  • MGC125976
  • POLB
  • polymerase (DNA directed), beta

Background

DNA polymerase beta comprises an amino-terminal 8 kD domain and a carboxy-terminal 31 kD domain.
DNA Polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Polymerase beta Antibody (NB600-1026)(2)

We have publications tested in 1 application: IB.


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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Bioinformatics

Gene Symbol POLB
Entrez