DNA Polymerase beta Antibody (18S)

Images

 
Western Blot: DNA Polymerase beta Antibody (18S) [NB600-1025] - Analysis of DNA Polymerase beta from Human Cells.
Immunoprecipitation: DNA Polymerase beta Antibody (18S) [NB600-1025] - Analysis of denatured IP.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ha, XpSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
18S
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DNA Polymerase beta Antibody (18S) Summary

Immunogen
Rat DNA polymerase beta protein
Localization
Nuclear.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
POLB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 2 ug/ml
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB600-1025 in the following applications:

Publications
Read Publications using
NB600-1025 in the following applications:

  • 1 publication
  • WB
    4 publications

Reactivity Notes

Clawed frog. Hamster reactivity reported in scientific literature (PMID: 14761848). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for DNA Polymerase beta Antibody (18S)

  • DNA pol beta
  • DNA polymerase beta subunit
  • DNA Polymerase beta
  • EC 2.7.7.7
  • EC 4.2.99.-
  • MGC125976
  • POLB
  • polymerase (DNA directed), beta

Background

DNA polymerase beta comprises an amino terminal 8kDa domain and a carboxy terminal 31kDa domain. DNA Polymerase beta, a DNA repair polymerase is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-1025
Species: Hu, Mu, Rt, Bv, Ha, Xp
Applications: WB, IHC, IHC-P, IP

Publications for DNA Polymerase beta Antibody (NB600-1025)(5)

Review for DNA Polymerase beta Antibody (NB600-1025) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-1025:
Filter by Applications
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Human
(1)
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Images Ratings Applications Species Date Details
 DNA Polymerase beta NB600-1025
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reviewed by:
Anonymous
Human 04/07/2017
View

Summary

Sample TestedLN428 cell gDNA
SpeciesHuman
LotRF2211901G

Comments

CommentsHuman cell line LN428 cells were treated by oxidative agent to induce DNA-protein cross-links (DPC) formation, genomic DNA (gDNA) were isolated, purified and blotted onto nitrocellulose membrane and probed for DNA polymerase signal.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DNA Polymerase beta Antibody (NB600-1025) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/07/2017
Application:
Species: Human

Bioinformatics

Gene Symbol POLB
Entrez
Uniprot