Western Blot: DNA Ligase III Antibody (6G9) [NB100-152] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [6G9] diluted at 1:1000. The signal was ...read more
Western Blot: DNA Ligase III Antibody (6G9) [NB100-152] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7. 5% SDS PAGE; antibody diluted at 1:1000.
ICC/IF usage reported in scientific literature. May be useful for IP active ligase. WB: Detects an approx. 95 kDa protein. Predicted MW is 103 kDa.
100 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
Immunogen affinity purified
Alternate Names for DNA Ligase III Antibody (6G9)
DNA ligase 3
DNA ligase III
ligase II, DNA, ATP-dependent
ligase III, DNA, ATP-dependent
Polydeoxyribonucleotide synthase [ATP] 3
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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