DNA Ligase III Antibody (6G9)

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Western Blot: DNA Ligase III Antibody (6G9) [NB100-152] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [6G9] diluted at 1:1000. The signal was ...read more
Western Blot: DNA Ligase III Antibody (6G9) [NB100-152] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Product Discontinued
View other related DNA Ligase III Primary Antibodies

Order Details


    • Catalog Number
      NB100-152
    • Availability
      Product Discontinued

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DNA Ligase III Antibody (6G9) Summary

Immunogen
Recombinant full-length human DNA Ligase III-b.
Localization
Mitochondrial and Nuclear
Specificity
This is specific for DNA ligase III.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LIG3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
ICC/IF usage reported in scientific literature. May be useful for IP active ligase. WB: Detects an approx. 95 kDa protein. Predicted MW is 103 kDa.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
DNA Ligase III Lysate (NBL1-12520)
Publications
Read Publications using
NB100-152 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Ligase III Antibody (6G9)

  • DNA ligase 3
  • DNA ligase III
  • EC 6.5.1.1
  • LIG2
  • ligase II, DNA, ATP-dependent
  • ligase III, DNA, ATP-dependent
  • Polydeoxyribonucleotide synthase [ATP] 3

Background

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Ligase III Antibody (NB100-152)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(3)
WB
(1)
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Filter By Species
Human
(3)
Rat
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA Ligase III Antibody (NB100-152) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Bioinformatics

Gene Symbol LIG3
Entrez
Uniprot