DNA Ligase I Antibody

Images

 
Immunohistochemistry-Paraffin: DNA Ligase I Antibody [NBP1-19701] - Paraffin-embedded human brain tissue.

Product Details

Summary
Product Discontinued
View other related DNA Ligase I Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19701
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

DNA Ligase I Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 110-160 of Human DNA Ligase I.
Clonality
Polyclonal
Host
Rabbit
Gene
LIG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of DNA Ligase I (E139) pAb in extracts from HT-29 cells.
Immunohistochemistry (IHC) analyzes of DNA Ligase I (E139) pAb in paraffin-embedded human brain tissue.
Theoretical MW
102125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Ligase I Antibody

  • DNA ligase 1
  • DNA ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-16182
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02280
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-41190
Species: Hu, Mu, Ch
Applications: WB, B/N, ICC/IF, IP, PLA
AF3495
Species: Hu
Applications: WB, IHC
NB100-119
Species: Hu, Mu
Applications: WB, ICC/IF, IP
AF3296
Species: Hu, Mu, Rt
Applications: WB
NB500-704
Species: Hu, Rt, ChHa
Applications: WB, ICC/IF, IHC, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP1-41190
Species: Hu, Mu, Ch
Applications: WB, B/N, ICC/IF, IP, PLA
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
NB100-74611
Species: Hu
Applications: WB, IHC-P, IP
NBP2-27335
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-38600
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-48053
Species: Hu
Applications: WB, IHC, IHC-P
NB100-477
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01020
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF3688
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-150
Species: Hu, Mu
Applications: WB, ICC/IF, IP
AF6349
Species: Hu, Mu, Rt
Applications: WB
NBP1-19701
Species: Hu
Applications: WB, IHC, IHC-P

Publications for DNA Ligase I Antibody (NBP1-19701) (0)

There are no publications for DNA Ligase I Antibody (NBP1-19701).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DNA Ligase I Antibody (NBP1-19701) (0)

There are no reviews for DNA Ligase I Antibody (NBP1-19701). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DNA Ligase I Antibody (NBP1-19701) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DNA Ligase I Products

Bioinformatics Tool for DNA Ligase I Antibody (NBP1-19701)

Discover related pathways, diseases and genes to DNA Ligase I Antibody (NBP1-19701). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DNA Ligase I Antibody (NBP1-19701)

Discover more about diseases related to DNA Ligase I Antibody (NBP1-19701).
 

Pathways for DNA Ligase I Antibody (NBP1-19701)

View related products by pathway.

PTMs for DNA Ligase I Antibody (NBP1-19701)

Learn more about PTMs related to DNA Ligase I Antibody (NBP1-19701).
 

Research Areas for DNA Ligase I Antibody (NBP1-19701)

Find related products by research area.

Blogs on DNA Ligase I.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DNA Ligase I Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol LIG1
Entrez