DNA Ligase I Antibody (7A12)

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Product Details

Summary
Product Discontinued
View other related DNA Ligase I Primary Antibodies

Order Details


    • Catalog Number
      NB100-2714
    • Availability
      Product Discontinued

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DNA Ligase I Antibody (7A12) Summary

Immunogen
Bovine DNA ligase I
Specificity
DNA Ligase I (7A12)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LIG1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunoprecipitation
Application Notes
By Western blot, this antibody detects a band of 125 kDa, which corresponds to the predicted molecular weight of DNA ligase I.
Control
HeLa Whole Cell Lysate
Publications
Read Publication using NB100-2714.

Reactivity Notes

Human and Cow.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.9% Sodium Azide
Purity
Protein A purified

Alternate Names for DNA Ligase I Antibody (7A12)

  • DNA ligase 1
  • DNA ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Ligase I Antibody (NB100-2714)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics Tool for DNA Ligase I Antibody (NB100-2714)

Discover related pathways, diseases and genes to DNA Ligase I Antibody (NB100-2714). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for DNA Ligase I Antibody (NB100-2714)

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Blogs on DNA Ligase I.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Bioinformatics

Gene Symbol LIG1
Entrez