DLL4 Antibody (4A11F8)

Images

 
Western Blot: DLL4 Antibody (4A11F8) [NBP2-22210] - Western blot analysis using DLL4 mAb against human DLL4 recombinant protein. (Expected MW is 39.2 kDa)
Immunohistochemistry-Paraffin: DLL4 Antibody (4A11F8) [NBP2-22210] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using DLL4 mouse mAb with DAB staining.
Western Blot: DLL4 Antibody (4A11F8) [NBP2-22210] - Western blot analysis using DLL4 mAb against HEK293 (1) and DLL4 (AA: 313-439)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: DLL4 Antibody (4A11F8) [NBP2-22210] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
4A11F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

DLL4 Antibody (4A11F8) Summary

Immunogen
Purified recombinant fragment of human DLL4 (AA: 313-439) expressed in E. Coli.
Localization
Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DLL4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
74.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for DLL4 Antibody (4A11F8)

  • Delta 4 precursor
  • delta 4
  • delta ligand 4
  • delta4
  • delta-like 4 (Drosophila)
  • delta-like 4 homolog (Drosophila)
  • delta-like 4 homolog
  • delta-like 4 protein
  • delta-like protein 4
  • DLL4
  • Drosophila Delta homolog 4
  • hdelta2
  • MGC126344
  • notch ligand delta-2
  • notch ligand DLL4

Background

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on DLL4.

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We at Novus Biologicals have a large antibody database devoted to signalling pathways. These underpin every area of molecular biological research, including cancer. Among our cell signalling antibodies is one targeted to DLL4 (Delta-like protein 4). D...  Read full blog post.

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Bioinformatics

Gene Symbol DLL4
Entrez