Rat Dkk-1 ELISA Kit (Colorimetric)

ELISA: Rat Dkk-1 ELISA Kit (Colorimetric) [NBP2-61303] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Rat Dkk-1 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 39.1 - 1250 pg/ml
• Sensitivity: 26.1 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: Mean 9.9%
• Intra-Assay: Mean 8.0%
• Spike Recovery: F12K + 10% FBS
  Low - 88%
  Medium - 96%
  High - 109%
  Serum Low - 77
  Medium - 101%
  High - 116%
  EDTA Plasma
  Low - 92%
  Medium - 98%
  High - 98%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DKK1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Rat Dkk-1 ELISA Kit (Colorimetric)

• dickkopf (Xenopus laevis) homolog 1
• dickkopf homolog 1 (Xenopus laevis)
• dickkopf related protein-1
• Dickkopf-1
• dickkopf-related protein 1
• Dkk1
• Dkk-1
• hDkk-1
• SKdickkopf-1 like

Background

Dkk-1 (Dickkopf-1) is a secreted inhibitor of the canonical Wnt signaling pathway, which mediates a variety of cellular processes including the growth and development of osteoblasts. Dkk-1 disrupts the canonical pathway by binding to the LRP5/6 co-receptors, preventing interaction with Wnt, and facilitating the degradation of beta-catenin. Dkk-1 is overexpressed in multiple myeloma, increasing bone resorption and the appearance of osteolytic lesions characteristic of multiple myeloma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Dkk-1 ELISA Kit (NBP2-61303)(1)

Publication using NBP2-61303

• Mace M, Gravesen E, Nordholm A et al. The Calcified Vasculature in Chronic Kidney Disease Secretes Factors that Inhibit Bone Mineralization JBMR Plus 2022-03-01 [PMID: 35434452] (ELISA)

FAQs for Dkk-1 ELISA Kit (NBP2-61303). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DKK1