Dishevelled-3 Antibody

Images

 
Western Blot: Dishevelled 3 Antibody [NBP2-16169] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Dishevelled 3 Antibody [NBP2-16169] - Immunofluorescence analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Dishevelled 3 Antibody [NBP2-16169] - Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.99 mg/ml

Order Details

Dishevelled-3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Dishevelled 3. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DVL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus tropicalis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.99 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dishevelled-3 Antibody

  • dishevelled 3 (homologous to Drosophila dsh)
  • dishevelled, dsh homolog 3 (Drosophila)
  • Dishevelled3
  • Dishevelled-3
  • DSH homolog 3
  • DVL3
  • KIAA0208dishevelled-3
  • segment polarity protein dishevelled homolog DVL-3

Background

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DVL3
Entrez
Uniprot