Recombinant Human DIMT1L Protein

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SDS-Page: Recombinant Human DIMT1L Protein [NBP1-98909] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human DIMT1L Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-313 of Human DIMT1L

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMPKVKSGAI GRRRGRQEQR RELKSAGGLM FNTGIGQHIL KNPLIINSII DKAALRPTDV VLEVGPGTGN MTVKLLEKAK KVVACELDPR LVAELHKRVQ GTPVASKLQV LVGDVLKTDL PFFDTCVANL PYQISSPFVF KLLLHRPFFR CAILMFQREF ALRLVAKPGD KLYCRLSINT QLLARVDHLM KVGKNNFRPP PKVESSVVRI EPKNPPPPIN FQEWDGLVRI TFVRKNKTLS AAFKSSAVQQ LLEKNYRIHC SVHNIIIPED FSIADKIQQI LTSTGFSDKR ARSMDIDDFI RLLHGFNAEG IHFS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DIMT1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 2 mM DTT, 200 mM NaCl, 2 mM EDTA
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human DIMT1L Protein

  • 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase
  • DIM1 dimethyladenosine transferase 1-like (S. cerevisiae)
  • DIM1 dimethyladenosine transferase 1-like
  • DIM1
  • DIMT1dimethyladenosine transferase
  • EC 2.1.1
  • EC 2.1.1.14
  • EC 2.1.1.183,18S rRNA dimethylase
  • HSA9761
  • HUSSY5
  • N'-adenosyl(rRNA) dimethyltransferase
  • probable dimethyladenosine transferase
  • S-adenosylmethionine-6-N'

Background

DIMT1 (DIM1 dimethyladenosine transferase 1 homolog) belongs to the methyltransferase superfamily. This enzyme is specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. It functions to dimethylate adjacent adenosines on the conserved hairpin loop of 18S rRNA in the 40S particle. DIMT1L is localized to the nucleolus. Recombinant human DIMT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DIMT1
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