Recombinant Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Protein

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SDS-Page: Recombinant Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Protein [NBP1-49442] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-285 of Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DDAH1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
33.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Protein

  • DDAH1
  • DDAH-1
  • DDAHdimethylargininase-1
  • DDAHI
  • Dimethylargininase-1
  • dimethylarginine dimethylaminohydrolase 1FLJ25539
  • EC 3.5.3.18
  • FLJ21264
  • N(G)
  • N(G)-dimethylarginine dimethylaminohydrolase 1
  • NG, NG-dimethylarginine dimethylaminohydrolase

Background

Dimethylarginine dimethylaminohydrolase 1, also known DDAH1, belongs to the dimethylarginine dimethylaminohydrolase gene family. The DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Impairment of DDAH1 causes ADMA (asymmetric dimethylarginine) accumulation and a reduction in cGMP generation. Recombinant human DDAH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Protein (NBP1-49442) (0)

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FAQs for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Protein (NBP1-49442). (Showing 1 - 1 of 1 FAQ).

  1. We have a customer who is looking for recombinant protein to use as positive control in western blot for eNOS/NOS3, eNOS/NOS3 phosphorylated on serine 1177, DDAH1 and DDAH2.
    • The use of recombinant proteins as positive controls on western blot is primarily driven by the antibody used. Here is a list of proteins we have available. A number of them are partial recombinant proteins and you would need to make sure that your customers immunogen region for the corresponding antibody he/she is using falls within the amino acid range for the recombinant proteins. eNOS: H00004846-Q01, DDAH1: H00023576-P01, NBP1-49442, DDAH2: H00023564-P01. We currently do not have a phosphorylated eNOS recombinant protein.

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Bioinformatics

Gene Symbol DDAH1
Entrez