Dihydrofolate Reductase/DHFR Antibody (6G7)

Images

 
Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Dihydrofolate reductase monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dihydrofolate reductase.
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.
Western Blot: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dihydrofolate reductase (Right lane) cDNA for 48 hrs and ...read more
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by ...read more
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody (6G7) [NBP1-47727] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Dihydrofolate reductase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
6G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Dihydrofolate Reductase/DHFR Antibody (6G7) Summary

Immunogen
Protein expressed in 293T cell transfected with human DHFR expression vector
Specificity
This antibody is specific for Homo sapiens dihydrofolate reductase (DHFR).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DHFR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Dihydrofolate Reductase/DHFR Antibody (6G7)

  • DHFR
  • DHFRP1
  • Dihydrofolate Reductase
  • DYR
  • EC 1.5.1.3

Background

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol DHFR
Entrez