DFF45/ICAD Antibody

Images

 
Western Blot: DFF45/ICAD Antibody [H00001676-D01P] - Analysis of DFFA expression in human pancreas.
Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody [H00001676-D01P] - Analysis of antibody to DFFA on HeLa cell. Antibody concentration 30 ug/ml.
Western Blot: DFF45/ICAD Antibody [H00001676-D01P] - Analysis of DFFA expression in A-431.
Western Blot: DFF45/ICAD Antibody [H00001676-D01P] - Analysis of DFFA expression in transfected 293T cell line by DFFA polyclonal antibody.Lane 1: DFFA transfected lysate(36.50 KDa).Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DFF45/ICAD Antibody Summary

Immunogen
DFFA (NP_004392.1, 1 a.a. - 331 a.a.) full-length human protein. MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DFFA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
It has been used for WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DFF45/ICAD Antibody

  • DFF1
  • DFF1DNA fragmentation factor 45 kDa subunit
  • DFF45
  • DFF45DNA fragmentation factor subunit alpha
  • DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
  • DFFA
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • ICAD
  • Inhibitor of CAD

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the Caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by Caspase 3 during apoptosis. A mouse homologue of human DFF was identified as inhibitor of caspase activated deoxyribonuclease (ICAD). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00001676-D01P
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Applications: WB, ICC/IF

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Bioinformatics

Gene Symbol DFFA
Entrez
Uniprot