DFF45/ICAD Antibody (3F12)

Images

 
Western Blot: ICAD Antibody (3F12) [NBP1-47725] Analysis of extracts (35ug) from 9 different cell lines by using anti-ICAD monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ICAD Antibody (3F12) [NBP1-47725] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ICAD.
Western Blot: ICAD Antibody (3F12) [NBP1-47725] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47725
    • Availability
      Product Discontinued

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DFF45/ICAD Antibody (3F12) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-331 of human DFF45
Specificity
This antibody is specific for Homo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DFFA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-500
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DFF45/ICAD Antibody (3F12)

  • DFF1
  • DFF1DNA fragmentation factor 45 kDa subunit
  • DFF45
  • DFF45DNA fragmentation factor subunit alpha
  • DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
  • DFFA
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • ICAD
  • Inhibitor of CAD

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DFFA
Entrez