Desmin Antibody

Images

 
Immunohistochemistry-Frozen: Desmin Antibody [NB120-15200] - Human liver section. Image from verified customer review.
Immunohistochemistry-Paraffin: Desmin Antibody [NB120-15200] - Human skeletal muscle stained with anti-desmin antibody.
Immunohistochemistry-Paraffin: Desmin Antibody [NB120-15200] - IHC analysis of Desmin in paraffin embedded and frozen E8-E11 mouse embryos. Image courtesy of product review submitted by Tara Rasmussen.

Product Details

Summary
Product Discontinued
View other related Desmin Primary Antibodies

Order Details


    • Catalog Number
      NB120-15200
    • Availability
      Product Discontinued

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Desmin Antibody Summary

Immunogen
Synthetic peptide mapping near the C-terminus of human desmin.
Localization
Cytoplasmic
Specificity
Desmin
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DES
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:75-1:200
Application Notes
IF use reported in literature (PMID 22427904). Immunohistochemistry-Frozen was reported in scientific literature. Use in Western blot reported in scientific literature (PMID 24481605)
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Desmin Lysate (NBL1-09840)
Skeletal Muscle Lysate (NB820-59253)
Leiomysarcoma Lysate (NB820-59430)
Reviewed Applications
Read 2 Reviews rated 5
using
NB120-15200 in the following applications:

Publications
Read Publications using
NB120-15200 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence homology: Bovine, Chicken, Dog, Frog, Pig and Rat, Canine, Porcine, Rabbit. Sheep reactivity reported in scientific literature (PMID: 25639519)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Desmin Antibody

  • CMD1I
  • CMD1IFLJ41013
  • CSM1
  • CSM2
  • DES
  • Desmin
  • FLJ12025
  • FLJ39719
  • FLJ41793
  • intermediate filament protein

Background

Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z bands giving a characteristic striated appearance. Antidesmin antibody is useful in identification of tumors of myogenic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmin Antibody (NB120-15200)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Sheep.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(1)
IHC-Fr
(2)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB120-15200 Applications Species
Singh BN, koyano-Nakagawa N, Gong W et al. A conserved HH-Gli1-Mycn network regulates heart regeneration from newt to human. Nat Commun. Oct 12 2018 [PMID: 30315164] (IHC-Fr, Mouse) IHC-Fr Mouse
Chen Y, Wu Z, Yuan B et al. MicroRNA-146a-5p attenuates irradiation-induced and LPS-induced hepatic stellate cell activation and hepatocyte apoptosis through inhibition of TLR4 pathway. Cell Death Dis. Jan 18 2018 [PMID: 29348414] (ICC/IF, Human) ICC/IF Human
Barnabei MS, Sjaastad FV, Townsend D et al. Severe dystrophic cardiomyopathy caused by the enteroviral protease 2A-mediated C-terminal dystrophin cleavage fragment Sci Transl Med. 2015 Jul 01 [PMID: 26136477]
Castillo-Melendez M, Yawno T, Allison BJ et al. Cerebrovascular adaptations to chronic hypoxia in the growth restricted lamb Int. J. Dev. Neurosci. 2015 Jan 29 [PMID: 25639519] (IHC-P, Sheep) IHC-P Sheep
Hirokawa Yumiko, Yip Kelvin Hon Yan, Tan Chin Wee, Burgess Antony W. Colonic myofibroblast cell line stimulates colonoid formation. Am J Physiol Gastrointest Liver Physiol. 2014 Apr 01 [PMID: 24481605] (WB, Mouse) WB Mouse
Wang WW, Wang W, Jiang Y et al. Reprogramming of mouse renal tubular epithelial cells to induced pluripotent stem cells. Cytotherapy 2013 Feb 14 [PMID: 23415920] (ICC/IF, Mouse) ICC/IF Mouse
Dembinski JL, Wilson SM, Spaeth EL et al. Tumor stroma engraftment of gene-modified mesenchymal stem cells as anti-tumor therapy against ovarian cancer Cytotherapy 2013 Jan [PMID: 23260083] (IHC-Fr, Mouse) IHC-Fr Mouse
Barnabei MS, Metzger JM. Ex Vivo Stretch Reveals Altered Mechanical Properties of Isolated Dystrophin-Deficient Hearts PLoS ONE 2012 [PMID: 22427904] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Koning M, Werker PM, van der Schaft DW et al. MicroRNA-1 and MicroRNA-206 Improve Differentiation Potential of Human Satellite Cells: A Novel Approach for Tissue Engineering of Skeletal Muscle Tissue Eng Part A 2012 May 18 [PMID: 22070522] (ICC/IF, Human) ICC/IF Human
Rasmussen TL, Kweon J, Diekmann MA et al. ER71 directs mesodermal fate decisions during embryogenesis. Development;138(21):4801-12. 2011 Nov. [PMID: 21989919]

Reviews for Desmin Antibody (NB120-15200) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-15200:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Desmin NB120-15200
Enlarge
reviewed by:
Margherita verardo
IHC-Fr Human 06/12/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedHuman liver
SpeciesHuman
Lot160407LVC
Immunohistochemistry-Paraffin Desmin NB120-15200
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 05/02/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedE11 mouse embryos
SpeciesMouse
CommentsThis is a great antibody. The example given is a fixed paraffin embedded slide with Dako antigen retrieval. However, it has also worked without AR, with other AR methods, and on cryosections. It has worked well in the heart for every method that I have attempted for E8-E11 mouse embryos.

Blocking

Blocking DetailsBlocked with IHC buffer for 1 hour at RT (IHC buffer: 2% normal donkey serum, 1% bovine serum albumin, 0.3% Triton X-100, 0.02%

Primary Anitbody

Dilution Ratio1:200 in IHC buffer, overnight at 4C

Secondary Antibody

Secondary DescriptionJackson ImmunoResearch donkey anti rabbit Cy3, 1:200, and Jackson ImmunoResearch donkey anti-Chicken-dylight 488,

Details

Detection NotesResults were imaged on a Zeiss Axio Imager M1 upright microscope
Fixation Details4% PFA, Antigen retrieval with Dako AR buffer in 95C water bath for 20 minutes, cooled at RT for 30 minutes
Wash DescriptionWashed with PBS 3 times for 5 minutes each

Comments

CommentsThis is a great antibody. The example given is a fixed paraffin embedded slide with Dako antigen retrieval. However, it has also worked without AR, with other AR methods, and on cryosections. It has worked well in the heart for every method that I have attempted for E8-E11 mouse embryos.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Desmin Antibody (NB120-15200) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Desmin Antibody (NB120-15200)

Discover related pathways, diseases and genes to Desmin Antibody (NB120-15200). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Desmin Antibody (NB120-15200)

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Pathways for Desmin Antibody (NB120-15200)

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PTMs for Desmin Antibody (NB120-15200)

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Research Areas for Desmin Antibody (NB120-15200)

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Blogs on Desmin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Margherita verardo
06/12/2018
Application: IHC-Fr
Species: Human

Anonymous
05/02/2012
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol DES
Entrez
Uniprot