Dectin-2/CLEC6A Antibody (545943) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
545943
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Dectin-2/CLEC6A Antibody (545943) [Alexa Fluor® 488] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin‑2/CLEC6A
Thr46-Leu209
Accession # Q6EIG7
Specificity
Detects human Dectin‑2/CLEC6A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Dectin-2 alpha, recombinant human (rh) CLEC9A, rhCLEC4D, or rhDLEC is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLEC6A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-2/CLEC6A Antibody (545943) [Alexa Fluor® 488]

  • CLEC4N
  • CLEC6A
  • CLECSF10
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10
  • C-type lectin domain family 6 member A
  • C-type lectin domain family 6, member A
  • C-type lectin superfamily member 10
  • DC-associated C-type lectin 2
  • dectin 2
  • Dectin2
  • Dectin-2
  • Dendritic cell-associated C-type lectin 2

Background

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4+ T cells (4‑6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is up‑regulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4+CD25+ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  3. Flornes, L.M. et al. (2004) Immunogenetics 56:506.
  4. Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522.
  5. Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281.
  6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
  7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
  8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
  9. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
  10. Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CLEC6A
Entrez
Uniprot