Dectin-2/CLEC6A Antibody (545943) [Alexa Fluor® 488] Summary
Mouse myeloma cell line NS0-derived recombinant human Dectin‑2/CLEC6A
Accession # Q6EIG7
Detects human Dectin‑2/CLEC6A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Dectin-2 alpha, recombinant human (rh) CLEC9A, rhCLEC4D, or rhDLEC is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Flow Cytometry 0.25-1 ug/10^6 cells
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
0.09% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Dectin-2/CLEC6A Antibody (545943) [Alexa Fluor® 488]
- C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10
- C-type lectin domain family 6 member A
- C-type lectin domain family 6, member A
- C-type lectin superfamily member 10
- DC-associated C-type lectin 2
- dectin 2
- Dendritic cell-associated C-type lectin 2
Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4+ T cells (4‑6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is up‑regulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4+CD25+ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).
- Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
- Kanazawa, N. et al. (2004) Immunobiology 209:179.
- Flornes, L.M. et al. (2004) Immunogenetics 56:506.
- Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522.
- Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281.
- Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
- McGreal, E.P. et al. (2006) Glycobiology 16:422.
- Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
- Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
- Aragane, Y. et al. (2003) J. Immunol. 171:3801.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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