Western Blot: DEC1 Antibody [NB100-1800] - Western Blot Image of anti-DEC1. Whole cell protein from A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: DEC1 Antibody [NB100-1800] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: DEC1 Antibody [NB100-1800] - P. gingivalis-challenged rat maxilla using DEC1 antibody at 1:100. Heat mediated antigen retrieval (sodium citrate buffer pH 6.0) was performed. ...read more
Flow Cytometry: DEC1 Antibody [NB100-1800] - An intracellular stain was performed on A431 cells with DEC1 Antibody NB100-1800AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Western Blot: DEC1 Antibody [NB100-1800] - Western blot analysis of DEC1 in HeLa whole cell lysate.
Independent Antibodies: Immunoprecipitation: DEC1 Antibody [NB100-1800] - Samples: Whole cell lysate from HeLa (50 ug for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit ...read more
Simple Western: DEC1 Antibody [NB100-1800] - Simple Western lane view shows a specific band for DEC1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Genetic Strategies: Immunoblotting: DEC1 Antibody [NB100-1800] - (B) HSC-2 cells were used to perform knockdown assays for three primary antibodies: HIF1A, DEC1, and DEC2 9. Real-time RT-PCR evaluated expression ...read more
Immunoblotting: DEC1 Antibody [NB100-1800] - DNA-DRR genes promoter activities decreased with DEC. (B) The DEC1 or DEC2 expression plasmids (B), each reporter, and DEC1 or delta basic DEC1 expression plasmids (C) were ...read more
Genetic Strategies: Immunoblotting: DEC1 Antibody [NB100-1800] - Knockdown assays in HSC-2 cells for HIF1A, DEC1, and DEC2. After transfecting and culturing non-specific siRNA or targeted siRNA into HSC-2, ...read more
This DEC1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen approx. 49 kDa. In ICC/IF punctate nuclear staining is observed.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 29715265). Use in Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID: 31061528). Use in ELISA reported in scientific literature (PMID: 31061528).
46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The basic helix-loop-helix (bHLH) transcription factor DEC1 (differentially expressed in chondrocytes protein 1) is wildly expressed in most normal tissues in a proportion of cells, whereas in tumors, its expression gets upregulated (potentially by hypoxia) in several malignancies such as breast, stomach, colon, lung, liver cancer and pancreatic ductal adenocarcinoma. The gene expression of DEC1 is regulated by various extracellular stimuli in a cell type-specific manner. Localized in the nucleus, it can homodimerize or heterodimerize with TCF3/E47, and interacts with ubiquitin-conjugating enzyme UBE2I/UBC9. DEC1 has emerged as one of the major circadian molecules alongwith its involvement in homeostasis of metabolism and energy. DEC1 has been shown to play a key role in various biological phenomena such as circadian rhythms, neurogenesis, neuroregulation, chondrogenesis, cellular growth, carcinogenesis, immune response balance as well as cellular senescence.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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