DDIT4 Antibody

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Western Blot: DDIT4 Antibody [NBP1-76382] - 293 cell lysate with DDIT4 antibody at (A) 2 and (B) 4 ug/mL.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-76382
    • Availability
      Product Discontinued

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DDIT4 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human RTP801. The immunogen is located within the first 50 amino acids of RTP801.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDIT4
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
RTP801 antibody can be used for the detection of RTP801 by Western blot at 2 - 4 mg/ml.
Control
DDIT4 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for DDIT4 Antibody

  • Dig2
  • DNA-damage-inducible transcript 4
  • FLJ20500
  • HIF-1 responsive RTP801 (RTP801)
  • Protein regulated in development and DNA damage response 1
  • REDD-1DNA damage-inducible transcript 4 protein
  • REDD1HIF-1 responsive protein RTP801
  • RTP801RP11-442H21.1

Background

RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recombinant Monoclonal Antibodies

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Bioinformatics

Gene Symbol DDIT4
Uniprot
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