DDB1 Antibody

Images

 
Western Blot: DDB1 Antibody [NB100-777] - (0.01ug/ml) staining of NIH3T3 (A) and NSO (B) lysate. (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: DDB1 Antibody [NB100-777] - (1ug/ml) staining of HeLa (A), HepG2 (B) and Jurkat (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DDB1 Antibody Summary

Immunogen
Peptide with sequence C-DLIKVVEELTRIH corresponding to C-Terminus according to NP_001914.3.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB:Approx 140kDa band observed in lysates of cell line HeLa, HepG2 and Jurkat. (calculated MW of 126.9kDa according to Human NP_001914.3). Recommended concentration: 1-2ug/ml. Approx 140kDa band observed in lysates of cell line NIH3T3 and approx. 150kDa band observed in lysates of cell line NSO (calculated MW of 126.8kDa according to Mouse NP_056550.1).
Publications
Read Publications using
NB100-777 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDB1 Antibody

  • damage-specific DNA binding protein 1 (127kD)
  • damage-specific DNA binding protein 1, 127kDa
  • Damage-specific DNA-binding protein 1
  • DDB p127 subunit
  • DDBA
  • DNA damage-binding protein 1
  • DNA damage-binding protein a
  • HBV X-associated protein 1
  • UV-damaged DNA-binding factor
  • UV-damaged DNA-binding protein 1
  • UV-DDB 1
  • UV-DDB1
  • XAP1
  • XAP-1
  • Xeroderma pigmentosum group E-complementing protein
  • XPCE
  • XPE
  • XPE-BF
  • XPE-binding factor

Background

Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDB1 Antibody (NB100-777)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DDB1
Entrez
Uniprot