DC-SIGN/CD209 Antibody (5H10) [Biotin]

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Flow Cytometry: DC-SIGN/CD209 Antibody (5H10) [Biotin] [NBP1-43567] - Staining of C57BL/6 splenocytes with 1.0 ug of Rat IgG2a kappa Isotype Control Biotin (open histogram) or 1.0 ug of Anti-Mouse CD209 (DC-SIGN) ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43567
    • Availability
      Product Discontinued

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DC-SIGN/CD209 Antibody (5H10) [Biotin] Summary

Immunogen
The immunogen for this antibody was CIRE.
Marker
Dendritic Cell Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD209
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This 5H10 antibody has been tested by immunoblotting and immunoprecipitation of CIRE in CIRE-transfected CHO cells. The reactivity of this antibody has been confirmed by immunoprecipitation of CIRE with 5H10 followed by immunoblotting with another CIRE-specific monoclonal antibody, LWC06 and, immunoprecipitation of CIRE with LWC06 followed by immunoblotting with 5H10.
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Reviewed Applications
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NBP1-43567 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for DC-SIGN/CD209 Antibody (5H10) [Biotin]

  • CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
  • CD209 molecule
  • CD209
  • CDSIGNHIV gpl20-binding protein
  • CLEC4L
  • CLEC4LC-type lectin domain family 4 member L
  • DCSIGN
  • DC-SIGN
  • DC-SIGN1
  • DC-SIGN1C-type lectin domain family 4, member L
  • DC-SIGNMGC129965
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

The 5H10 antibody was generated by immunization with a peptide derived from the extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca2+ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The 5H10 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DC-SIGN/CD209 Antibody (NBP1-43567) (0)

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Review for DC-SIGN/CD209 Antibody (NBP1-43567) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-43567:
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Images Ratings Applications Species Date Details
Western Blot DC-SIGN/CD209 NBP1-43567
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reviewed by:
Stephen Bunnell
WB Mouse 08/08/2016
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Summary

ApplicationWestern Blot
Sample TestedHEK293T cell lysate
SpeciesMouse
LotE028131632

Blocking

Blocking Details5%BSA in TBST

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat Anti-Rat
Secondary Concentration1:10,000

Details

Detection NotesChemiluminescence, HRP conjugated secondary antibody, 5 Minute exposure

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Secondary Antibodies

 

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Diseases for DC-SIGN/CD209 Antibody (NBP1-43567)

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PTMs for DC-SIGN/CD209 Antibody (NBP1-43567)

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Recent Reviews

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1
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Stephen Bunnell
08/08/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CD209
Entrez