DC-SIGN/CD209 Antibody (5H10)


Western Blot: DC-SIGN/CD209 Antibody (5H10) [NBP1-43329] - CIRE/DC-SIGN-transfected (left) and untransfected control CHO cell lysates were loaded at 1x10^5 cells/lane, probed with 1 ug/mL of Anti-Mouse CD209 (DC-SIGN) ...read more
Flow Cytometry: DC-SIGN/CD209 Antibody (5H10) [NBP1-43329] - Analysis using the Biotin conjugate of NBP1-43329. Staining of C57BL/6 splenocytes with 1.0 ug of Rat IgG2a kappa Isotype Control Biotin (open histogram) or ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IP
0.5 mg/ml

DC-SIGN/CD209 Antibody (5H10) Summary

The immunogen for this antibody was CIRE.
Dendritic Cell Marker
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Flow Cytometry
  • Immunoprecipitation 1:10-1:500
Application Notes
This 5H10 antibody has been tested by immunoblotting and immunoprecipitation of CIRE in CIRE-transfected CHO cells. The reactivity of this antibody has been confirmed by immunoprecipitation of CIRE with 5H10 followed by immunoblotting with another CIRE-specific monoclonal antibody, LWC06 and, immunoprecipitation of CIRE with LWC06 followed by immunoblotting with 5H10.

Alternate Names for DC-SIGN/CD209 Antibody (5H10)

  • CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
  • CD209 molecule
  • CD209
  • CDSIGNHIV gpl20-binding protein
  • CLEC4L
  • CLEC4LC-type lectin domain family 4 member L
  • DC-SIGN1
  • DC-SIGN1C-type lectin domain family 4, member L
  • DC-SIGNMGC129965
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 1


The 5H10 antibody was generated by immunization with a peptide derived from the extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca2+ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The 5H10 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP1-43567
PE Labeled NBP2-00329

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Gene Symbol CD209

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