Daxx Antibody (7A11)

Product Discontinued

Daxx Antibody (7A11) Summary

• Immunogen: Purified recombinant fragment of human DAXX expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAXX
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1: 500-1:2000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 50% glycerol
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Daxx Antibody (7A11)

• BING2
• CENP-C binding protein
• DAP6
• DAP6MGC126245
• Daxx
• death domain-associated protein 6
• death-associated protein 6
• death-domain associated protein
• EAP1
• ETS1-associated protein 1
• Fas death domain-associated protein
• Fas-binding protein
• hDaxx
• MGC126246

Background

Apoptosis, or programmed cell death, occurs duringnormal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED3 protease family. A novel DD containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx is a death domain containing important intermediate in the Fas mediated apoptosis. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N terminal kinase (JNK) pathway. It is widely expressed in fetal and adult human and mouse tissue, indicating its important function in Fas signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAXX