Cytokeratin, Acidic (Type I or LMW) Antibody (AE1)

Flow (Intracellular): Cytokeratin, Acidic (Type I or LMW) Antibody (AE1) [NBP2-62221] - Intracellular staining of CaCo-2 cells with anti-acidic cytokeratins (AE1) PE.

• Reactivity: Hu, Mu, Rt, Ca, Ch, Fe, Pm, Rb
• Applications: WB, Flow, Flow-IC, IHC, IHC-Fr, IHC-P, ICC/IF
• Clone: AE1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cytokeratin, Acidic (Type I or LMW) Antibody (AE1) Summary

• Immunogen: Human epidermal keratins
• Specificity: The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1-5 ug/ml
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.