Cytokeratin 8 Antibody (4D12)

Images

 
Western Blot: Cytokeratin 8 Antibody (4D12) [NBP2-02089] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (4D12) [NBP2-02089] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (4D12) [NBP2-02089] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytokeratin 8 mouse monoclonal antibody.
Western Blot: Cytokeratin 8 Antibody (4D12) [NBP2-02089] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (4D12) [NBP2-02089] - Immunofluorescent staining of A549 cells using anti-Cytokeratin 8 mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-02089
    • Availability
      Product Discontinued

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Cytokeratin 8 Antibody (4D12) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
Marker
Epithelial Cell Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Dog, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 8 Antibody (4D12)

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

Background

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on Cytokeratin 8.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT8
Entrez