Cytokeratin 7 Antibody (K72.7)

Images

 
Western Blot: Cytokeratin 7 Antibody (K72.7) [NBP2-44816] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (K72.7).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (K72.7) [NBP2-44816] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling KRT7 with Cytokeratin 7 Antibody (K72.7) followed by Goat anti-Mouse ...read more
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) [NBP2-44816] - Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (K72.7)
Flow Cytometry: Cytokeratin 7 Antibody (K72.7) [NBP2-44816] - Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 7 Antibody (K72.7)followed by Goat anti-Mouse IgG-CF488 (blue); Isotype Control (red).
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) [NBP2-44816] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (K72.7)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
K72.7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 7 Antibody (K72.7) Summary

Immunogen
Semi-Purified cytokeratin preparation.
Localization
Cytoplasmic
Marker
Glandular and Transitional Epithelial Marker
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47941)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 7 Antibody (K72.7)

  • CK7
  • CK-7
  • cytokeratin 7
  • Cytokeratin-7
  • K2C7
  • K7keratin, 55K type II cytoskeletal
  • keratin 7
  • keratin, type II cytoskeletal 7
  • keratin-7
  • MGC129731
  • MGC3625
  • Sarcolectin
  • SCLkeratin, simple epithelial type I, K7
  • type II mesothelial keratin K7
  • Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT7
Uniprot