Cytokeratin 7 Antibody (4F5)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42297
    • Availability
      Product Discontinued

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Cytokeratin 7 Antibody (4F5) Summary

Immunogen
The antibody reacts with the Cytokeratin 7 antigen.
Specificity
The antibody recognizes cytokeratins nos. 7, 17 and 19 typical for many simple epithelial cells but is found also in cells in squamous epithelia. Monoclonal antibody to cytokeratin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified cytokeratins from cultured pig kidney epithelial cells were used as immunogen.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT7
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Immunohistochemical stainings (frozen sections) and western blotting.Working dilution in western blotting is at least 1:1000.

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS solution containing 1.0% w/v BSA
Preservative
0.1% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (4F5)

  • CK7
  • CK-7
  • cytokeratin 7
  • cytokeratin-7
  • K2C7
  • K7keratin, 55K type II cytoskeletal
  • keratin 7
  • keratin, type II cytoskeletal 7
  • keratin-7
  • MGC129731
  • MGC3625
  • sarcolectin
  • SCLkeratin, simple epithelial type I, K7
  • type II mesothelial keratin K7
  • Type-II keratin Kb7

Background

Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT7