Cytokeratin 19 Antibody (RCK108) - Unpurified

Images

 
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Immunofluorescence staining of a 9 days old zebrafish embryo
Immunohistochemistry: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Rat
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Immunohistochemistry on frozen section of human liver bile duct epithelium
Immunohistochemistry: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Human Liver
Immunohistochemistry: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Human Tongue
Immunohistochemistry: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Rat Liver
Immunohistochemistry: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Rat Liver
Immunohistochemistry: Cytokeratin 19 Antibody (RCK108) [NBP1-42238] - Rat

Product Details

Summary
Reactivity Hu, Rt, ZeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
RCK108
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Unpurified

Cytokeratin 19 Antibody (RCK108) - Unpurified Summary

Immunogen
The antibody reacts with the Keratin 19 antigen.
Localization
Cytoplasm
Marker
Epithelial Cell Marker
Specificity
The monoclonal antibody specifically reacts with the cytokeratin 19, a 40 kDa polypeptide, which is found in most simple and non-keratinized stratified epithelia. This antibody reacts on formalin fixed, paraffin embedded material and can be used in diagnostic immunopathology. The reaction pattern of cytokeratin 19 is documented in the literature. Antigen origin: human bladder cell line T24. Antigen location: Cytoplasm.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT19
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Flow Cytometry 1:10-1:20
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunohistochemistry 1:10-1:100
  • Immunohistochemistry-Frozen 1:10-1:100
  • Immunohistochemistry-Paraffin 1:10-1:100
Application Notes
Immunohistochemistry on frozen sections. Strongly positive on epithelial structures known to express keratin 19 in acetone fixed sections. Immunohistochemistry on paraffin embedded sections. Strongly positive with formaldehyde fixed sections. Dilution: 1:10 - 1:20 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution).
Publications
Read Publication using
NBP1-42238 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Cytokeratin 19 Antibody (RCK108) - Unpurified

  • CK19
  • CK-19
  • Cytokeratin 19
  • Cytokeratin-19,40-kDa keratin intermediate filament
  • EndoC
  • K19
  • K19cytokeratin-19
  • K1CS
  • keratin 19
  • keratin, type I cytoskeletal 19
  • keratin, type I, 40-kd
  • keratin-19
  • Krt19
  • MGC15366

Background

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. (2-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 19 is the smallest human keratin, is found in many simple and some non-keratinizing stratified squamous epithelia, and is often co-expressed with keratin 7. (7,8) Cytokeratin 19 has been implicated as a marker for stem cells in some tissues, such as hair follicles in the skin. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. The degree of cytokeratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Cytokeratin 19 is also associated with malignant transformation in esophageal and pancreatic cancers. (9,10)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 19 Antibody (NBP1-42238)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: ICC/IF, IHC.


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(1)
IHC
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT19
Entrez
Uniprot