Cytokeratin 18 Antibody

Images

 
Western Blot: Cytokeratin 18 Antibody [NBP2-16090] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 18 Antibody [NBP2-16090] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody [NBP2-16090] - Immunohistochemical analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.

Product Details

Summary
Product Discontinued
View other related Cytokeratin 18 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-16090
    • Availability
      Product Discontinued

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Cytokeratin 18 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.
Localization
Cytoplasm; perinuclear region
Marker
Epithelial Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT18
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.83 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 18 Antibody

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Cytokeratin 18 Antibody (NBP2-16090)

Discover related pathways, diseases and genes to Cytokeratin 18 Antibody (NBP2-16090). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Cytokeratin 18 Antibody (NBP2-16090)

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Research Areas for Cytokeratin 18 Antibody (NBP2-16090)

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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18
Entrez
Uniprot