Cytokeratin 18 [p Ser33] Antibody

Images

 
Western Blot: Keratin 18 Phosphospecific Antibody [NB100-92571] - Western blot (WB) analysis of p-Keratin 18 (S33) antibody in extracts from HT29 cells.
Immunohistochemistry: Keratin 18 Phosphospecific Antibody [NB100-92571] - Immunohistochemistry (IHC) analyzes of p-Keratin 18 (S33) antibody in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related Cytokeratin 18 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92571
    • Availability
      Product Discontinued

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Cytokeratin 18 [p Ser33] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of Serine 33.
Modification
p Ser33
Marker
Epithelial Marker
Clonality
Polyclonal
Host
Rabbit
Gene
KRT18
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Cytokeratin 18 (S33) pAb in extracts from HT29.
Immunohistochemistry (IHC) analyzes of Cytokeratin 18 (S33) pAb in paraffin-embedded human breast carcinoma.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human,.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 18 [p Ser33] Antibody

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Background

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18
Entrez
Uniprot