Cytokeratin 18 Antibody (M9)

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Product Details

Summary
Product Discontinued
View other related Cytokeratin 18 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-42139
    • Availability
      Product Discontinued

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Cytokeratin 18 Antibody (M9) Summary

Immunogen
Cytokeratin isolated from human breast carcinoma cell line MCF-7.
Marker
Epithelial Marker
Specificity
In immunoblotting experiments the antibody recognizes only keratin no. 18 (45 kD) and lacks cross reactivity with other cytokeratins. cross reactivity GFAP : not detectable Neurofilament : not detectable Vimentin : not detectable Desmin : not detectable
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT18
Purity
Tissue culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
The antibody stains especially simple epithelia. Frozen sections can be stained with immuno-per­oxidase and immu­nofluor­escence tests. A positive result on paraffin embedded tissues could be obtained with a TUF (cat.no. T 10333) preteatment at a antibodyWorking dilution for frozen sections approximately 1:150, preferably in phosphate buffered saline. See for useful procedures instructions G and I.

Reactivity Notes

Human,Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
No Preservative
Purity
Tissue culture supernatant

Alternate Names for Cytokeratin 18 Antibody (M9)

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on Cytokeratin 18.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18
Entrez