Cytokeratin 14 Antibody

Images

 
Western Blot: Cytokeratin 14 Antibody [NBP2-16087] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP2-16087] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.64 mg/ml

Order Details

Cytokeratin 14 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.64 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 14 Antibody

  • CK14
  • CK-14
  • Cytokeratin 14
  • cytokeratin-14
  • EBS3
  • EBS4
  • K14
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14
  • keratin, type I cytoskeletal 14
  • keratin-14
  • KRT14
  • NFJ

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT14
Entrez
Uniprot