Cytokeratin 10 Antibody (LH2)

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Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (LH2) [NB100-2779] - Staining of human Bladder Carcinoma wtih Cytokeratin 10 Monoclonal Antibody (LH2).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
LH2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Cytokeratin 10 Antibody (LH2) Summary

Immunogen
extract of skin from psoriasis patient
Localization
Cytoplasmic
Specificity
LH2 is monospecific and has high affinity.Cellular Localization: CytoplasmicSuggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1 ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2 ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT10
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Only tested in human keratins. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

Positive control(s): Extract of skin from psoriasis patient.

Alternate Names for Cytokeratin 10 Antibody (LH2)

  • BCIE
  • BIE
  • CK10
  • CK-10
  • cytokeratin 10
  • Cytokeratin-10
  • EHK
  • K10keratosis palmaris et plantaris
  • Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris)
  • keratin 10
  • keratin, type I cytoskeletal 10
  • keratin-10
  • KPP

Background

Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KRT10
Entrez