Cytochrome P450 2E1 Antibody (5B9)

Images

 
Western Blot: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2E1(Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2E1.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of paraffin-embedded Ovary tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Immunofluorescent staining of HepG2 cells using anti-Cytochrome P450 2E1mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (5B9) [NBP2-02155] - Staining of paraffin-embedded endometrium tissue using anti-Cytochrome P450 2E1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
5B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Cytochrome P450 2E1 Antibody (5B9) Summary

Immunogen
Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYP2E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytochrome P450 2E1 Antibody (5B9)

  • 4-nitrophenol 2-hydroxylase
  • CPE1
  • CYP2Ecytochrome P450 2E1
  • CYPIIE1
  • cytochrome P450, family 2, subfamily E, polypeptide 1
  • cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
  • Cytochrome P450-J
  • EC 1.14.13.-
  • EC 1.14.13.n7
  • EC 1.14.14.1
  • flavoprotein-linked monooxygenase
  • microsomal monooxygenase
  • P450C2E
  • P450-J
  • xenobiotic monooxygenase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CYP2E1
Entrez
Uniprot