Recombinant Human Cystatin E/M/CST6 Protein

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SDS-Page: Cystatin E/M/CST6 Recombinant Protein [NBP2-52263] - 15% SDS Page (3 ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
0.5 mg/ml

Order Details

Recombinant Human Cystatin E/M/CST6 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 29-149 of Human Cystatin E/M/CST6

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM

Specificity
The IC50 value is < 10nM. The inhibitory function of Cystat6 on protease activity of papawas measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
Details of Functionality
The IC50 value is < 10nM. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CST6
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
15.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Alternate Names for Recombinant Human Cystatin E/M/CST6 Protein

  • CST6
  • cystatin 6
  • Cystatin E/M
  • cystatin M
  • cystatin M/E
  • Cystatin-6
  • cystatin-E
  • cystatin-M
  • cysteine proteinase inhibitor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CST6
Entrez