Cystatin C Antibody (Cyst28)

Images

 
Immunoassay: Cystatin C Antibody (Cyst28) [NBP1-05765] - Calibration curves: One-step fluoroimmunoassay in streptavidin coated plates. Capture MAbs: Cyst24 and Cyst23 are biotinylated (200 ng/well). Detection MAbs: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IA, S-ELISA
Clone
Cyst28
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Cystatin C Antibody (Cyst28) Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with cystatin C purified from human urine.
Specificity
Detects human Cystatin C, a member of the type 2 cystatin superfamily. Cystatin C is a potent inhibitor of cysteine proteinases, both endogenous (lysosomal cathepsines), and pathogenic.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CST3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoassay
  • Sandwich ELISA
Application Notes
Recommended pairs for sandwich immunoassay (capture-detection): Cyst24 - Cyst19, (NB100-62127/NBP1-05766) Cyst24 - Cyst28, (NB100-62127/NBP1-05765) Cyst23 - Cyst13, (NB100-62126/NBP1-05763). Recommended pairs with the best cross-reaction with animal sera (capture-detection): Cyst11 - Cyst20 (dog serum); Cyst29 - Cyst16 (cat serum); Cyst29 - Cyst20 (cat serum); Cyst29 - Cyst20 (horse serum); Cyst20 - Cyst13 (horse serum).
Control
Cystatin C Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Cystatin C Antibody (Cyst28)

  • ARMD11
  • bA218C14.4 (cystatin C)
  • CST3
  • cystatin 3
  • cystatin C (amyloid angiopathy and cerebral hemorrhage)
  • Cystatin C
  • cystatin-3
  • cystatin-C
  • Gamma-trace
  • MGC117328
  • Neuroendocrine basic polypeptide
  • Post-gamma-globulin

Background

Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. The cystatin superfamily encompasses proteins that contain multiple cystatin like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. Cystatin C (Cystatin 3) , a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight of 13260 Da, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CST3
Entrez