Cystatin B/Stefin B Antibody (2F1)

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Western Blot: Cystatin B/Stefin B Antibody (2F1) [NBP1-04283] - The Cell lysates of A549 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CSTB (1:1000). Proteins were visualized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Cystatin B/Stefin B Antibody (2F1) Summary

Immunogen
Recombinant human CSTB (1-98aa) purified from E. coli
Specificity
Anti-human CSTB mAb, clone 2F1, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CSTB protein.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CSTB
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cystatin B/Stefin B Antibody (2F1)

  • CPI-B
  • CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B
  • CSTB
  • cystatin B (stefin B)
  • Cystatin B
  • EPM1
  • Liver thiol proteinase inhibitor
  • PME
  • Stefin B
  • stefin-B

Background

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to from a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes in cells. CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CSTB
Entrez
Uniprot