CYP7B1 Antibody (7E5)

Images

 
Western Blot: CYP7B1 Antibody (7E5) [NBP1-47722] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYP7B1 monoclonal antibody.
Western Blot: CYP7B1 Antibody (7E5) [NBP1-47722] - CYP7B1 antibody (7E5) at 1:500 dilution + lysates from HEK-293T transfected with human CYP7B1 expression vector.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
7E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CYP7B1 Antibody (7E5) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 350-506 of human Cyp7b1
Specificity
This antibody is specific for Homo sapiens cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CYP7B1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-500

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYP7B1 Antibody (7E5)

  • CBAS3
  • CP7B
  • Cytochrome P450 7B1
  • cytochrome P450, family 7, subfamily B, polypeptide 1
  • cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1,25-hydroxycholesterol 7-alpha-hydroxylase
  • EC 1.14.13.100
  • Oxysterol 7-alpha-hydroxylase
  • oxysterol 7alpha-hydroxylase
  • spastic paraplegia 5A (autosomal recessive)
  • SPG5A

Background

Cyp7b1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYP7B1
Entrez