CYLD Antibody

Product Discontinued

CYLD Antibody Summary

• Immunogen: Peptide with sequence C-DSQQSKSKNPWYIDE corresponding to internal region according to Uniprot Mouse NP_775545.1.
• Epitope: DSQQSKSKNPWYIDE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CYLD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 125 kDa band observed in mouse and rat brain lysates (calculated MW of 107 kDa band according to mouse NP_775545.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CYLD Antibody

• BRSS
• CDMT
• CYLD
• CYLD1
• CYLD1MFT
• CYLDI
• cylindromatosis (turban tumor syndrome)
• Deubiquitinating enzyme CYLD
• EAC
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ20180
• FLJ31664
• KIAA0849FLJ78684
• MFT1
• probable ubiquitin carboxyl-terminal hydrolase CYLD
• SBS
• TEM
• ubiquitin carboxyl-terminal hydrolase CYLD
• ubiquitin specific peptidase like 2
• ubiquitin thioesterase CYLD
• Ubiquitin thiolesterase CYLD
• Ubiquitin-specific-processing protease CYLD
• USPL2

Background

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYLD Antibody (NB100-58966)(1)

Publication using NB100-58966

• Massoumi R, Chmielarska K, Hennecke K et al. Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling. Cell 2006-05-19 [PMID: 16713561]

Bioinformatics

• Gene Symbol: CYLD
• Entrez:
  • 1540(Human)
  • 74256(Mouse)
  • 312937(Rat)
• Uniprot:
  • NP_775545.1(Mouse)