Cyclin E2 Antibody

Images

 
Western Blot: Cyclin E2 Antibody [NBP1-51283] - analysis of Cyclin E2 (N387) pAb in extracts from Jurkat cell.
Immunohistochemistry-Paraffin: Cyclin E2 Antibody [NBP1-51283] - analyzes of Cyclin E2 (N387) pAb in paraffin-embedded human thyroid gland tissue.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1.0 mg/ml

Order Details

Cyclin E2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 361-410 of Human Cyclin E2.
Specificity
Cyclin E2 (N387) pAb detects endogenous levels of Cyclin E2 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
CCNE2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Cyclin E2 (N387) pAb in extracts from Jurkat cell'.
Immunohistochemistry (IHC) analyzes of Cyclin E2 (N387) pAb in paraffin-embedded human thyroid gland tissue.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse

Alternate Names for Cyclin E2 Antibody

  • CCNE2
  • CYCE2
  • Cyclin E2
  • G1/S-specific cyclin E2
  • G1/S-specific cyclin-E2

Background

Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Cyclin E2 is a cyclin E-related protein that specifically interacts with Cdk2 and Cdk3 and with p27 and p21. Cyclin E2 expression peaks at the G1/S phase transition of the cell cycle, in parallel with cyclin E. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol CCNE2
Entrez

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