Cyclin E1 Antibody



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Cyclin E1 Antibody Summary

This antibody was generated by immunizing host with Peptide-KLH (GVLTPPHSSKKQSSEQETE)
Nucleus protein, ubiquitous expressed
Immunogen affinity purified
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  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 1:100~400 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.2)
0.09% Sodium Azide
Immunogen affinity purified

Alternate Names for Cyclin E1 Antibody

  • CCNE
  • CCNE1
  • CCNEcyclin Es
  • Cyclin E1
  • cyclin Et
  • G1/S-specific cyclin-E1


This rabbit polyclonal antibody recognizes human, mouse, and rat cyclin E also known as Cyclin E1, CCNE, G1/S specific cyclin E1. Cycline E is a nucleus protein, predicted molecular weight 47 kD. Cycline E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cycline E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E is essential for the control of the cell cycle at the G1/S transition. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. The poly6307 antibody has been shown to be useful for Western blotting, Immunoprecipitation, and Immunohistochemistry. Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol CCNE1