Cyclin D3 Antibody

Images

 
Western Blot: Cyclin D3 Antibody [NB100-92026] - Western blot (WB) analysis of Cyclin D3 (G277) antibody in extracts from COS7 cells treated with Forskolin 40nM 30'.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92026
    • Availability
      Product Discontinued

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Cyclin D3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 249-299 of Human Cyclin D3.
Clonality
Polyclonal
Host
Rabbit
Gene
CCND3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Cyclin D3 (G277) pAb in extracts from COS7 cells.
Immunohistochemistry (IHC) analyzes of Cyclin D3 (G277) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyclin D3 Antibody

  • CCND3
  • Cyclin D3
  • D3-type cyclin
  • G1/S-specific cyclin D3
  • G1/S-specific cyclin-D3

Background

The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCND3
Entrez