Cyclin D1 Antibody (CD1.1)

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
Clone
CD1.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cyclin D1 Antibody (CD1.1) Summary

Immunogen
Human Cyclin D1
Specificity
Recognizes the Cyclin D1, a 36kD nuclear protein. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy, and over expression has been linked to poor prognosis.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCND1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Flow Cytometry Neat
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:10000
  • Immunoprecipitation 1:10-1:500
Application Notes
Membrane permeabilization is required for Flow Cytometry. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Positive Control
Cyclin D1 Lysate (NBL1-08871)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Cyclin D1 Antibody (CD1.1)

  • B-cell lymphoma 1 protein
  • BCL-1 oncogene
  • Bcl-1
  • BCL1D11S287E
  • CCND1
  • cyclin D1 (PRAD1: parathyroid adenomatosis 1)
  • Cyclin D1
  • Cyl-1
  • G1/S-specific cyclin D1
  • G1/S-specific cyclin-D1
  • PRAD1 oncogene
  • PRAD1
  • PRAD1B-cell CLL/lymphoma 1
  • U21B31

Background

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. In a recent study, Cyclin D1 regulates cellular metabolism, fat cell differentiation and cellular migration. Cyclin D1 is also involved in development and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Beta-catenin - I am versatile!
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Bioinformatics

Gene Symbol CCND1
Entrez
Uniprot