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Cyclin B2 Antibody (X29.2) - BSA Free

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Product Details

Summary
Reactivity Ma, XpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
X29.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Cyclin B2 Antibody (X29.2) - BSA Free Summary

Immunogen
Xenopus laevis cyclin B2
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCNB2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
This antibody is Cytof ready.
Control
Cyclin B2 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Notes

X29.2 cross reacts with B1.

Positive control(s): Xenopus laevis testis.

Alternate Names for Cyclin B2 Antibody (X29.2) - BSA Free

  • CCNB2
  • Cyclin B2
  • G2/mitotic-specific cyclin-B2
  • HsT17299

Background

Cyclin B2 belongs to a the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin B2 has been shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCNB2
Entrez