Cyclin B1 Antibody (1B10)

Images

 
Western Blot: Cyclin B1 Antibody (1B10) [NBP1-51662] - Western blot analysis using CCNB1 mouse mAb against Hela (1), JURKET (2), K562 (3) and PC-12 (4) cell lysate.
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1B10) [NBP1-51662] - Immunohistochemical analysis of paraffin-embedded lung cancer (left) and ovary tumour tissues (right) using CCNB1 mouse mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
1B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Cyclin B1 Antibody (1B10) Summary

Immunogen
Purified recombinant fragment of human CCNB1 expressed in E. Coli.
Marker
Mitosis Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCNB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Cyclin B1 Antibody (1B10)

  • CCNB
  • CCNB1
  • Cyclin B1
  • G2/mitotic-specific cyclin B1
  • G2/mitotic-specific cyclin-B1

Background

In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCNB1
Entrez