CYB5R3 Antibody (2A10)

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Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody.
Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody, and then ...read more
Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human Kidney tissue using anti-CYB5R3 mouse monoclonal antibody.
Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - Analysis of Jurkat cells, using anti-CYB5R3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
2A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CYB5R3 Antibody (2A10) Summary

Immunogen
Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CYB5R3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-500
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human, Dog, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYB5R3 Antibody (2A10)

  • B5RNADH-cytochrome b5 reductase 3
  • cytochrome b5 reductase 3
  • Cytochrome b5 reductase
  • DIA1NADH-cytochrome b5 reductase 3 membrane-bound form
  • diaphorase (NADH) (cytochrome b-5 reductase)
  • diaphorase-1
  • EC 1.6.2.2
  • NADH-cytochrome b5 reductase 3 soluble form

Background

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CYB5R3
Entrez