CXCR2/IL-8 RB Antibody (48311) [Unconjugated]

Images

 
Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more
CXCR2/IL‑8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was ...read more
Recombinant Human CXCL1/GRO alpha (Catalog #275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
48311
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CXCR2/IL-8 RB Antibody (48311) [Unconjugated] Summary

Additional Information
Clone 48311 was used by HLDA to establish CD designation
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880
Specificity
Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CXCR2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Neutralization 1-5 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
MAB331 in the following applications:

Publications
Read Publications using
MAB331 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8 RB Antibody (48311) [Unconjugated]

  • beta
  • CD182 antigen
  • CD182
  • CDw128b
  • chemokine (C-X-C motif) receptor 2
  • chemokine (CXC) receptor 2
  • C-X-C chemokine receptor type 2
  • CXCR2 gene for IL8 receptor type B
  • CXCR2
  • CXC-R2
  • CXCR-2
  • GRO/MGSA receptor
  • High affinity interleukin-8 receptor B
  • IL-8 receptor type 2
  • IL-8R B
  • IL8R2
  • IL8RA
  • IL8RB
  • IL-8RB
  • interleukin 8 receptor B
  • interleukin 8 receptor type 2
  • interleukin-8 receptor type B

Background

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR2/IL-8 RB Antibody (MAB331)(42)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Guinea Pig.

We have publications tested in 12 applications: ELISA Development, Flow, ICC, IHC, IHC - Paraffin embedded, IHC Fresh, IHC Frozen, IHC Paraffin-embedded, IHC-P, In vivo, Neut, WB.


Filter By Application
ELISA Development
(1)
Flow
(9)
ICC
(1)
IHC
(2)
IHC - Paraffin embedded
(1)
IHC Fresh
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(2)
IHC-P
(1)
In vivo
(1)
Neut
(18)
WB
(4)
All Applications
Filter By Species
Human
(37)
Mouse
(3)
Rat
(1)
Guinea Pig
(1)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using MAB331 Applications Species
P Mazzuca, S Marsico, K Schulze, S Mitola, MC Pils, C Giagulli, CA Guzman, A Caruso, F Caccuri Role of autophagy in the HIV-1 matrix protein p17-driven lymphangiogenesis J. Virol., 2017;0(0):. 2017 [PMID: 28592537] (Neut,, Human) Neut Human
H Kasashima, M Yashiro, H Nakamae, G Masuda, H Kinoshita, T Morisaki, T Fukuoka, T Hasegawa, T Nakane, M Hino, K Hirakawa, M Ohira Clinicopathologic significance of the CXCL1-CXCR2 axis in the tumor microenvironment of gastric carcinoma PLoS ONE, 2017;12(6):e0178635. 2017 [PMID: 28575019] (IHC,, Human) IHC Human
ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients Respir. Res., 2017;18(1):100. 2017 [PMID: 28532454] (Flow,, Human) Flow Human
A Qaqish, D Huang, CY Chen, Z Zhang, R Wang, S Li, E Yang, Y Lu, MH Larsen, WR Jacobs, L Qian, J Frencher, L Shen, ZW Chen Adoptive Transfer of Phosphoantigen-Specific ?? T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates J. Immunol., 2017;0(0):. 2017 [PMID: 28526681] (Flow,, Human) Flow Human
WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression Nat Commun, 2017;8(0):14399. 2017 [PMID: 28186102] (Neut,, Human) Neut Human
Nader Alaridah Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs J Innate Immun, 2016;0(0):. 2016 [PMID: 28013312] (Neut,, Mouse) Neut Mouse
Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016;0(0):. 2016 [PMID: 27496101] (Flow,, Human) Flow Human
Kuang-Hui Sun TNF-? augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma J Cell Mol Med, 2016;0(0):. 2016 [PMID: 27297979] (WB,, Human) WB Human
Li L, Xu L, Yan J, Zhen Z, Ji Y, Liu C, Lau W, Zheng L, Xu J CXCR2-CXCL1 axis is correlated with neutrophil infiltration and predicts a poor prognosis in hepatocellular carcinoma. J Exp Clin Cancer Res, 2015;34(0):129. 2015 [PMID: 26503598] (IHC - Paraffin embedded,, Human) IHC - Paraffin embedded Human
Al-Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol, 2015;193(3):1416-26. 2015 [PMID: 24981451] (Neut,, Human) Neut Human
Show All 42 Publications.

Reviews for CXCR2/IL-8 RB Antibody (MAB331) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB331:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22763101
SpeciesHuman
Special ApplicationsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IHC-P Human 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22626766
SpeciesHuman
Special ApplicationsIndependent review from antibodyresource.com

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FAQs for CXCR2/IL-8 RB Antibody (MAB331) (0)

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Secondary Antibodies

 

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Diseases for CXCR2/IL-8 RB Antibody (MAB331)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
02/20/2015
Application: IHC-P
Species: Human

 
Anonymous
02/20/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CXCR2
Entrez
Uniprot