CXCR1/IL-8 RA Antibody

Images

 
Western Blot: CXCR1/IL-8 RA Antibody [NBP2-16043] - Untreated (-) and treated (+) mouse tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR1 antibody [C2C3], C-term (CFA = ...read more
Immunocytochemistry/ Immunofluorescence: CXCR1 Antibody [NBP2-16043] - Immunofluorescence analysis of methanol-fixed Hep G2, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CXCR1/IL-8 RA Antibody [NBP2-16043] - CXCR1 Antibody [NBP2-16043] - Detects IL8RA protein at cytosol on Hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded Hepatoma. CXCR1 ...read more
Western Blot: CXCR1 Antibody [NBP2-16043] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: CXCR1/IL-8 RA Antibody [NBP2-16043] - CXCR1 antibody [C2C3], C-term detects CXCR1 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: CXCR1 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.89 mg/ml
   

Biological Strategies

     

Order Details

CXCR1/IL-8 RA Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CXCR1. The exact sequence is proprietary.
Localization
Cell membrane
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CXCR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-16043 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.89 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CXCR1/IL-8 RA Antibody

  • CD128
  • CD181 antigen
  • CD181
  • CDw128aC-C
  • chemokine (C-X-C motif) receptor 1
  • CKR-1
  • CMKAR1
  • C-X-C chemokine receptor type 1
  • CXCR1
  • CXC-R1
  • CXCR-1
  • High affinity interleukin-8 receptor A
  • IL-8 receptor type 1
  • IL-8R A
  • IL8R1
  • IL8RA
  • IL-8RA
  • IL8RAC-C-CKR-1
  • IL8RBA
  • interleukin 8 receptor, alpha
  • interleukin-8 receptor type 1
  • interleukin-8 receptor type A

Background

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for CXCR1/IL-8 RA Antibody (NBP2-16043) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-16043:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot CXCR1/IL-8 RA NBP2-16043
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reviewed by:
Anonymous
WB Human 09/11/2014
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Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
CommentsThe blot on the website shows a single band at approx 70 KDa (CXCR1 is 41 KDa). In 293T cells, I got a band at 41KDa and another faint one at 70 KDa.

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:1000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-rabbit IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#111-005-045
Secondary Concentration1:20000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Comments

CommentsThe blot on the website shows a single band at approx 70 KDa (CXCR1 is 41 KDa). In 293T cells, I got a band at 41KDa and another faint one at 70 KDa.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CXCR1/IL-8 RA Antibody (NBP2-16043) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
09/11/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CXCR1
Entrez
Uniprot