Recombinant Human CXCL8/IL-8 Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42918
    • Availability
      Product Discontinued

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Recombinant Human CXCL8/IL-8 Protein Summary

Description
A partial protein corresponding to IL8.
Preparation
Method
E. coli expressed amino acids Ser 29-Ser 99 of human IL-8 (accession # NM_000584).
Details of Functionality
Measured by chemotaxis assay of human peripheral blood neutrophils. Maximal chemoattractant activity was observed between 10-100 ng/ml.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IL8
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

Application Notes
This recombinant human IL-8 has been tested in bioassays for chemoattractant activity, with maximal activity between 10-100 ng/ml.

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with no carrier or stabilizer. 0.22 uM filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant Human CXCL8/IL-8 Protein

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAPGCP1
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

Human IL-8, also called neutrophil attractant/activating protein (NAP-1), monocyte derived neutrophil activating peptide (MONAP), monocyte derived neutrophil activating factor (ANF), monocyte derived neutrophil chemotactic factor (MDNCF), and neutophil activating factor (NAF), is a ~6-8 kDa factor produced by variety types of cells including monocytes, lymphocytes, granulocytes, fibroblasts and endothelial cells. IL-8 is an inflammatory cytokine which functions as a neutrophil chemoatractant and activating factor. It also attracts basophils and a subpopulation of lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL8
Entrez