Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [DyLight 405]

Images

 
There are currently no images for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P
Clone
HECA-452
Clonality
Monoclonal
Host
Rat

Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [DyLight 405] Summary

Immunogen
Human tonsil stroma
Specificity
Cutaneous Lymphocyte Antigen
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Rat
Gene
SELPLG
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 405
Format
DyLight 405 (A=400, E=420)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
IgM purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes. Purity is > 85%. IHC use reported in literature (see Duijvestijn AM, et al).

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [DyLight 405]

  • CD162 antigen
  • CD162
  • CLA
  • Cutaneous Lymphocyte-Associated Associated Antigen
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • Selectin P Ligand

Background

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-42795
Species: Hu
Applications: WB, Flow, Func, IHC, IHC-Fr, IP
NBP1-61399
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NB200-610
Species: Hu, Ca, GP
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
NBP2-14921
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-03286
Species: Hu, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-01743
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-1831
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-78039V
Species: Hu, Mu
Applications: Flow, IHC, IHC-Fr, IHC-P

Publications for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V) (0)

There are no publications for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V) (0)

There are no reviews for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-78039APC
DyLight 350 Labeled NB100-78039UV
DyLight 405LS Labeled NB100-78039V3
DyLight 488 Labeled NB100-78039G
DyLight 680 Labeled NB100-78039FR
DyLight 755 Labeled NB100-78039IR
PE Labeled NB100-78039PE
PerCP Labeled NB100-78039PCP

Additional Cutaneous Lymphocyte Antigen (CLA) Products

Cutaneous Lymphocyte Antigen (CLA) NB100-78039V

Related Products by Gene

Bioinformatics Tool for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V)

Discover related pathways, diseases and genes to Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V)

Discover more about diseases related to Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V).
 

Pathways for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V)

View related products by pathway.

PTMs for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V)

Learn more about PTMs related to Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V).
 

Research Areas for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039V)

Find related products by research area.

Blogs on Cutaneous Lymphocyte Antigen (CLA).

CLA - guiding T-cells to sites of inflammation
T-cells infiltrating sites of inflammation of the skin typically express the cutaneous lymphocyte-associate antigen (CLA). This antigen is defined by the binding of the monoclonal CLA antibody HECA-452. The CLA antigen is a fucose-containing oligo...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol SELPLG

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-78039V below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

CD11b Antibody
NB110-89474
CD11b Antibody
NB110-89474
CD11b Antibody
NB110-89474