Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452)

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Western Blot: Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml.
Immunohistochemistry-Paraffin: Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody ...read more
Flow (Cell Surface): Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a ...read more
Flow Cytometry: Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by anti-mouse IgG FITC
Flow (Cell Surface): Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, Flow-CS, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
HECA-452
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) Summary

Immunogen
Human tonsil stroma
Specificity
Cutaneous Lymphocyte Antigen
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Rat
Gene
SELPLG
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA
  • Flow Cytometry < = 2 ug/10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • CyTOF-ready
  • Flow (Cell Surface)
Positive Control
SR-BI Lysate (NBL1-15720)
Publications
Read Publications using
NB100-78039 in the following applications:

  • 1 publication
  • 2 publications
  • IHC
    2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Notes

CLA antibody clone HECA-452 is known to recognize the cutaneous lymphocyte-associated antigen / CLA which is a specialized glycosylated form of P-selectin glycoprotein ligand-1/PSGL-1. Besides lymphocytes, this clone has been shown to react with normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).

Alternate Names for Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452)

  • CD162 antigen
  • CD162
  • CLA
  • Cutaneous Lymphocyte-Associated Associated Antigen
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • Selectin P Ligand

Background

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ELISA, FLOW, IHC, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
IHC
(2)
WB
(2)
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Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-78039 Applications Species
Kim CH, Cheong KA, Park CD, Lee AY. Therapeutic Effects of Combination Using Glucosamine Plus Tacrolimus (FK-506) on The Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice. Scand J Immunol;75(5):471-8. 2012 May. [PMID: 22023698] (IHC, Mouse) IHC Mouse
Yamanaka K, Dimitroff CJ, Fuhlbrigge RC et al. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression. J Allergy Clin Immunol. 2008 Jan [PMID: 17910894] (WB) WB
Walcheck B, Leppanen A, Cummings R et al. The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin. Blood. 2002 Jun 1 [PMID: 12010808] (ELISA, FLOW) ELISA, FLOW
Borges E, Pendl G, Eytner R et al. The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated. J Biol Chem. 1997 Nov. [PMID: 9353350]
Duijvestijn AM, Horst E, Pals ST et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am J Pathol. 1988 Jan. [PMID: 3276207]
Berg EL, Yoshino T, Rott LS et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med. 1991 Dec. [PMID: 1720810]
Picker LJ, Kishimoto TK, Smith CW et al. ELAM-1 is an adhesion molecule for skin-homing T cells. Nature. 1991 Feb. [PMID: 1705666]
Picker LJ, Michie SA, Rott LS, Butcher EC. A unique phenotype of skin-associated lymphocytes in humans. Preferential expression of the HECA-452 epitope by benign and malignant T cells at cutaneous sites. Am J Pathol. 1990 May [PMID: 1693467] (FLOW, WB, IHC) FLOW, WB, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-78039APC
DyLight 350 Labeled NB100-78039UV
DyLight 405 Labeled NB100-78039V
DyLight 488 Labeled NB100-78039G
DyLight 680 Labeled NB100-78039FR
DyLight 755 Labeled NB100-78039IR
PE Labeled NB100-78039PE
PerCP Labeled NB100-78039PCP

Additional Cutaneous Lymphocyte Antigen (CLA) Products

Cutaneous Lymphocyte Antigen (CLA) NB100-78039

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Diseases for Cutaneous Lymphocyte Antigen (CLA) Antibody (NB100-78039)

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Blogs on Cutaneous Lymphocyte Antigen (CLA).

CLA - guiding T-cells to sites of inflammation
T-cells infiltrating sites of inflammation of the skin typically express the cutaneous lymphocyte-associate antigen (CLA). This antigen is defined by the binding of the monoclonal CLA antibody HECA-452. The CLA antigen is a fucose-containing oligo...  Read full blog post.

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Bioinformatics

Gene Symbol SELPLG